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Goat Anti-CARD11 Antibody (EB05886)

Related Products: EBP05886 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05886 Goat Anti-CARD11 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $423.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05886 1
EB05886 staining (1µg/ml) of Human Jurkat cells (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
EB05886 2
EB05886 (2µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CARD11, caspase recruitment domain family, member 11, BIMP3, CARMA1, MGC133069, card-maguk protein 1, bcl10-interacting maguk protein 3
Official Symbol: CARD11
Accession Number(s): NP_115791.3
Human GeneID(s): 84433
Non-Human GeneID(s): 108723 (mouse)

Immunogen

Peptide with sequence C-QRKTIWVDEDQL, from the C Terminus of the protein sequence according to NP_115791.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 150+65kDa bands observed in lysates of cell line Jurkat (calculated MW of 133kDa according to NP_115791.2). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide.
IHC: In paraffin embedded Human Spleen shows nuclear staining. Recommended concentration: 2-5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog, Cow

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