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Goat Anti-CHRNA7 Antibody (EB08853)

Related Products: EBP08853 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08853 Goat Anti-CHRNA7 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human, Rat $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08853 1
EB08853 (5ug/ml) staining of paraffin embedded Rat Cerebral Cortex (A) and Rat Hippocampus (B), CRY-staining with DAPI nuclear counter stain in blue. Antigen retrieval with citrate buffer pH 6 at 95-98C.
EB08853 2
EB08853 (3µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CHRNA7, cholinergic receptor, nicotinic, alpha 7, NACHRA7, a7 nicotinic acetylcholine receptor, alpha 7 neuronal nicotinic acetylcholine receptor, alpha-7 nicotinic cholinergic receptor subunit, alpha7 nicotinic receptor subunit, cholinergic receptor, nicotinic, alpha polypeptide 7, neuronal acetylcholine receptor protein, alpha-7 chain, neuronal nicotinic acetylcholine receptor alpha-7 subunit, nicotinic acetylcholine alpha-7 subunit, nicotinic acetylcholine receptor alpha7 subunit, nicotinic cholinergic receptor alpha 7
Official Symbol: CHRNA7
Accession Number(s): NP_000737.1
Human GeneID(s): 1139
Non-Human GeneID(s): 11441 (mouse), 25302 (rat)
Important Comments: This antibody also reacts with CHRNA7-FAM7A fusion isoform 1 (NP_647536.1) and isoform 2 (NP_683709.1).

Immunogen

Peptide with sequence KRPGEDKVRPACQHKQ, from the internal region of the protein sequence according to NP_000737.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
IHC: In paraffin embedded Rat Cerebral Cortex and Rat Hippocampus shows membranous staining in neural cells. Recommended concentration: 5-10µg/ml. Paraffin embedded Human Brain (Cortex). Recommended concentration: 3-6µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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