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Goat Anti-CREM Antibody (EB06532)

Related Products: EBP06532 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06532 Goat Anti-CREM Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB06532 1

EB06532 optimised QC. Primary incubation 1 hour at room temperature.

Image A,: Human Heart lysate at primary Ab concentration 3µg/ml. (Loaded 35µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: CREM, ICER, MGC17881, MGC41893, MGC111110, hCREM-2, cAMP responsive element modulator, hCREM 2alpha-b protein, hCREM 2beta-a protein, inducible cAMP early repressor ICER, OTTHUMP00000019442, OTTHUMP00000019443, cAMP response element modulator; hCREM 2alpha-b protein
Official Symbol: CREM
Accession Number(s): NP_853549.1; NP_874387.1; NP_874388.1; NP_874393.1; NP_874394.1; NP_877570.1; NP_877571.1; NP_877572.1; NP_877573.1; NP_878270.1; NP_898883.1
Human GeneID(s): 1390
Important Comments: This antibody is expected to recognise the following protein isoforms of human CREM protein: a (NP_853549.1); e (NP_874387.1); f (NP_874388.1); k (NP_874393.1); l (NP_874394.1); m (NP_877570.1); n (NP_877571.1); o (NP_877572.1); p (NP_877573.1); q (NP_878270.1); v (NP_898883.1). This antibody is not expected to cross-react with CREB protein.

Immunogen

Peptide with sequence LETLKDICSPKTDY, from the C Terminus of the protein sequence according to NP_853549.1; NP_874387.1; NP_874388.1; NP_874393.1; NP_874394.1; NP_877570.1; NP_877571.1; NP_877572.1; NP_877573.1; NP_878270.1; NP_898883.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 35-37kDa band observed in Human Heart lysates, and in preliminary testing of Human Kidney lysate (calculated MW of 32.5kDa according to Human NP_853549.1). Recommended concentration: 2-3µg/ml. Primary incubation 1 hour at room temperature.

A customer reported a band at approx. 35-37kDa in Rat Heart lysates after 3ug/ml primary ab concentration (calculated MW of 38.5kDa according to NP_001258030.1). Primary incubation 1 hour at room temperature.


Species Reactivity

Tested: Human
Expected from sequence similarity: Human