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Goat Anti-CRKL Antibody (EB05254)

Related Products: EBP05254 - Immunizing Peptide, Goat Anti-CRKL Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB05254 Goat Anti-CRKL Antibody Pep-ELISA, WB, IHC, IF, FC Yes 100µg specific antibody in 200µl Human, Mouse $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05254 1
EB05254 (0.1µg/ml) staining of K562 (A) and (1ug/ml) Daudi (B) and NIH3T3 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05254 2
EB05254 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB05254 3
EB05254 Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB05254 4
EB05254 (3.8µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CRKL, v-crk sarcoma virus CT10 oncogene homolog (avian)-like, v-crk avian sarcoma virus CT10 oncogene homolog-like, OTTHUMP00000198539
Official Symbol: CRKL
Accession Number(s): NP_005198.1
Human GeneID(s): 1399

Immunogen

Peptide with sequence C-KIFDPQNPDENE, from the C Terminus of the protein sequence according to NP_005198.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 37kDa band observed in lysates of cell lines K562, Daudi and NIH3T3 (calculated MW of 33.8kDa according to Human NP_005198.1 and Mouse NP_031790.2 ). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Thymus shows staining of cellular membranes in thymocytes. Recommended concentration: 2.5-3.8µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat

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