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Goat Anti-CUL2 Antibody (EB10058)

Related Products: EBP10058 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10058 Goat Anti-CUL2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10058 1
EB10058 (1µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10058 2
EB10058 (3.75µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: cullin 2, MGC131970, OTTHUMP00000019437, CUL2
Official Symbol: CUL2
Accession Number(s): NP_003582.2
Human GeneID(s): 8453
Non-Human GeneID(s): 71745 (mouse), 361258 (rat)

Immunogen

Peptide with sequence C-QYIERSQASADE, from the C Terminus of the protein sequence according to NP_003582.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 100kDa band observed in lysates of cell line NIH3T3 (calculated MW of 87.0kDa according to Human NP_003582.2 and of 86.9kDa according to Mouse NP_083678.2). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Breast. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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