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Goat Anti-DUSP6 / MKP3 Antibody (EB11892)

Related Products: EBP11892 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11892 Goat Anti-DUSP6 / MKP3 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11892 1
EB11892 (0.3µg/ml) staining of Human (A), (Mouse) and Rat (C) Heart lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11892 2
EB11892 (0.3µg/ml) staining of Pig Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names:
Official Symbol: DUSP6
Accession Number(s): NP_001937.2; NP_073143.2
Human GeneID(s): 1848
Non-Human GeneID(s): 67603 (mouse), 116663 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001937.2; NP_073143.2).

Immunogen

Peptide with sequence C-PSNQNVYQVDSLQST, from the C Terminus of the protein sequence according to NP_001937.2; NP_073143.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 26kDa band observed in Mouse and Rat Heart lysates and 40kDa plus 26kDa bands observed in Human and Pig Heart lysates (calculated MW of 42.3kDa according to NP_001937.2 and 26.5kDa according to NP_073143.2). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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