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Goat Anti-ELMO1 Antibody (EB05297)

Code Name Applications Availability Size Tested species Price Grade  
EB05297 Goat Anti-ELMO1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB05297 1
EB05297 (0.3µg/ml) staining of Human Frontal Cortex (A), Mouse (B) and Rat (C) Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05297 2
EB05297 (0.3µg/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ELMO1, engulfment and cell motility 1 (ced-12 homolog, C. elegans), CED12, KIAA0281, ced-12 homolog 1, engulfment and cell motility 1, CED-12, ELMO-1, MGC126406, OTTHUMP00000207056, OTTHUMP00000207060
Official Symbol: ELMO1
Accession Number(s): NP_055615.8; NP_569709.1; NP_001034548.1
Human GeneID(s): 9844
Non-Human GeneID(s): 140580 (mouse)
Important Comments: This antibody is expected to recognise both human isoforms of this protein. NP_569709.1 and NP_001034548.1 represent the same isoform.

Immunogen

Peptide with sequence PKEPSNYDFVYDCN, from the C Terminus of the protein sequence according to NP_055615.8; NP_569709.1; NP_001034548.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 83kDa band observed in Human Brain (Frontal Cortex) lysates, in Mouse and Rat Brain lysates and in lysates of cell line Jurkat (calculated MW of 83.8kDa according to NP_055615.8). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.

Immunoprecipitation: A customer found positive results in IP on Human promyelocytic leukemia cells (HL60). This data is from a previous batch, not on sale.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Zebrafish

Specific References

This antibody (previous batch) has been successfully used in Western blot in Zebrafish:
Krishna Rakesh Sharma, Karl Heckler, Sandra J. Stoll, Jan-Luuk Hillebrands, Katharina Kynast, Esther Herpel, Stefan Porubsky, Marlies Elger, Boris Hadaschik, Karen Bieback, Hans-Peter Hammes, Peter P. Nawroth, and Jens Kroll
ELMO1 protects renal structure and ultrafiltration in kidney development and under diabetic conditions.
Sci Rep. 2016 Nov 16;6:37172
PMID: 27849017

This antibody (previous batch) has been successfully used in Western blot in Human:
Schäker K, Bartsch S, Patry C, Stoll SJ, Hillebrands JL, Wieland T, Kroll J.
The bipartite rac1 Guanine nucleotide exchange factor engulfment and cell motility 1/dedicator of cytokinesis 180 (elmo1/dock180) protects endothelial cells from apoptosis in blood vessel development.
J Biol Chem. 2015 Mar 6;290(10):6408
PMID: 25586182

This antibody (previous batch) has been successfully used in Western blot and IP in Zebrafish:
Epting D, Wendik B, Bennewitz K, Dietz CT, Driever W, Kroll J.
The Rac1 regulator ELMO1 controls vascular morphogenesis in zebrafish.
Circ Res. 2010 Jul 9;107(1):45-55.
PMID: 20466982

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