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|EB05297||Goat Anti-ELMO1 Antibody||Pep-ELISA, WB||100µg specific antibody in 200µl||Human, Mouse, Rat||$324.00|
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Peptide with sequence PKEPSNYDFVYDCN, from the C Terminus of the protein sequence according to NP_055615.8; NP_569709.1; NP_001034548.1.
Please note the peptide is available for sale.
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Immunoprecipitation: A customer found positive results in IP on Human promyelocytic leukemia cells (HL60). This data is from a previous batch, not on sale.
This antibody (previous batch) has been successfully used in Western blot in Zebrafish:
Krishna Rakesh Sharma, Karl Heckler, Sandra J. Stoll, Jan-Luuk Hillebrands, Katharina Kynast, Esther Herpel, Stefan Porubsky, Marlies Elger, Boris Hadaschik, Karen Bieback, Hans-Peter Hammes, Peter P. Nawroth, and Jens Kroll
ELMO1 protects renal structure and ultrafiltration in kidney development and under diabetic conditions.
Sci Rep. 2016 Nov 16;6:37172
This antibody (previous batch) has been successfully used in Western blot in Human:
Schäker K, Bartsch S, Patry C, Stoll SJ, Hillebrands JL, Wieland T, Kroll J.
The bipartite rac1 Guanine nucleotide exchange factor engulfment and cell motility 1/dedicator of cytokinesis 180 (elmo1/dock180) protects endothelial cells from apoptosis in blood vessel development.
J Biol Chem. 2015 Mar 6;290(10):6408
This antibody (previous batch) has been successfully used in Western blot and IP in Zebrafish:
Epting D, Wendik B, Bennewitz K, Dietz CT, Driever W, Kroll J.
The Rac1 regulator ELMO1 controls vascular morphogenesis in zebrafish.
Circ Res. 2010 Jul 9;107(1):45-55.