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Code Name Applications Availability Size Tested species Price Grade  
EB09815 Goat Anti-MICS1 / GHITM Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09815 1
EB09815 (0.01µg/ml) staining of Human Cerebellum (A), Duodenum (B) and Heart (C) cell lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09815 2
EB09815 (0.3µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: dermal papilla-derived protein 2, DERP2, DKFZp566C0746, FLJ26584, growth hormone inducible transmembrane protein, HSPC282, MICS1, mitochondrial morphology and cristae structure 1, My021, PTD010, TMBIM5, transmembrane BAX inhibitor motif containing, GHITM
Official Symbol: GHITM
Accession Number(s): NP_055209.2
Human GeneID(s): 27069
Non-Human GeneID(s): 66092 (mouse), 290596 (rat)

Immunogen

Peptide with sequence C-PSREYATKTRIGIR, from the internal region of the protein sequence according to NP_055209.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 35kDa band observed in lysates of cell line HeLa and of Human Brain (Cerebellum), Duodenum and Heart (calculated MW of 37.2kDa according to Human NP_055209.2). Recommended concentration: 0.01-0.1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig

Specific Reference

This antibody has been successfully used in Western blot on Human:
Meng H, Yamashita C, Shiba-Fukushima K, Inoshita T, Funayama M, Sato S, Hatta T, Natsume T, Umitsu M, Takagi J, Imai Y, Hattori N.
Loss of Parkinson’s disease-associated protein CHCHD2 affects mitochondrial crista structure and destabilizes cytochrome c
Nat Commun. 2017 Jun 7;8:15500.
PMID: 28589937

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