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Goat Anti-GPI / Neuroleukin Antibody (EB09739)

Code Name Applications Availability Size Tested species Price Grade  
EB09739 Goat Anti-GPI / Neuroleukin Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB09739 1
EB09739 (0.01µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09739 2
EB09739 (3.75µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB09739 3
EB09739 (3.75µg/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB09739 4
EB09739 (3.75µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: GPI, glucose phosphate isomerase, AMF, GNPI, NLK, PGI, SA-36, autocrine motility factor;, glucose-6-phosphate isomerase, hexose monophosphate isomerase, hexosephosphate isomerase;, neuroleukin, oxoisomerase;, phosphoglucose isomerase, phosphohexomutase, phosphohexose isomerase, phosphosaccharomutase, sperm antigen-36
Official Symbol: GPI
Accession Number(s): NP_000166.2
Human GeneID(s): 2821

Immunogen

Peptide with sequence C-YREHRSELNLRR, from the internal region (near N Terminus) of the protein sequence according to NP_000166.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 60kDa band observed in Human Heart lysates (calculated MW of 63.1kDa according to NP_000166.2). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was hour.
IHC: Paraffin embedded Human Testis, Thyroid and Kidney. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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