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Goat Anti-HEY2 Antibody (EB10135)

Related Products: EBP10135 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10135 Goat Anti-HEY2 Antibody Pep-ELISA, WB, IF, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10135 1

EB10135 optimised QC. Primary incubation 1 hour at room temperature.

Image A: HeLa nuclear cell lysate at primary Ab concentration 3.5ug/ml. Image B: HEK293 nuclear cell lysate at primary Ab concentration 2ug/ml. (Loaded 35µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
EB10135 2

EB10135 optimised QC. Primary incubation 1 hour at room temperature.

Image A: HEK293 cell lysate at primary Ab concentration 2ug/ml. (Loaded 35µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
EB10135 3
EB10135 Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB10135 4
EB10135 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: bHLHb32, cardiovascular basic helix-loop-helix factor 1, CHF1, GRIDLOCK, GRL, hairy/enhancer-of-split related with YRPW motif 2, hairy-related transcription factor 2, HERP1, HESR2, HES-related repressor protein 1, HRT2, MGC10720, OTTHUMP00000017138, HEY2
Official Symbol: HEY2
Accession Number(s): NP_036391.1
Human GeneID(s): 23493
Non-Human GeneID(s): 15214 (mouse), 155430 (rat)

Immunogen

Peptide with sequence HASESTPCRLSTTS, from the internal region of the protein sequence according to NP_036391.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 30kDa band observed in nuclear lysates of cell lines HeLa and HEK293 and in HEK293 whole cell lysate (calculated MW of 35.8kDa according to NP_036391.1). Recommended concentration: 2-3.5µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HEK293 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Cow

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