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Goat Anti-KLF8 / BKLF3 Antibody (EB05469)

Related Products: EBP05469 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05469 Goat Anti-KLF8 / BKLF3 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05469 1
EB05469 staining (2µg/ml) of Human Kidney lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
EB05469 2
EB05469 (3.75µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: KLF8, BKLF3, Kruppel-like factor 8, RP13-1021K9.1, DKFZp686O08126, DXS741, MGC138314, ZNF741, zinc finger protein 741, basic kruppel-like factor 3
Official Symbol: KLF8
Accession Number(s): NP_009181.2; NP_001152768.1; NP_001311029.1
Human GeneID(s): 11279
Important Comments: This antibody is expected to recognise isoform 1 (NP_009181.2) and isoform 2 (NP_001152768.1).

Immunogen

Peptide with sequence VDMDKLINNLEVQ-C, from the N Terminus of the protein sequence according to NP_009181.2; NP_001152768.1; NP_001311029.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 50kDa band observed in Human Kidney lysates (calculated MW of 39.3 acording to NP_009181.2) . In glioblastomas this protein was reported to run at appr. 60kDa (Ding et al . J Biol Chem. 2005 Feb 25;280(8):6802-15; PMID: 15557280). Recommended concentration: 1-3µg/ml.
IHC: Paraffin embedded Human Small Intestine. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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