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Goat Anti-Lipocalin 2 / NGAL Antibody (EB06762)

Related Products: EBP06762 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06762 Goat Anti-Lipocalin 2 / NGAL Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06762 1
EB06762 (0.03µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06762 2
EB06762 (2.5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: NGAL, lipocalin 2 (oncogene 24p3), HGNC:6526, LCN2, uterocalin, oncogenic lipocalin 24p3, neutrophil gelatinase-associated lipocalin, lipocalin 2, MSFI, OTTHUMP00000022240, migration-stimulating factor inhibitor, neutrophil gelatinase-associated lipocalin, oncogene 24p3, siderocalin
Official Symbol: LCN2
Accession Number(s): NP_005555.2
Human GeneID(s): 3934

Immunogen

Peptide with sequence C-AILREDKDPQKMY, from the internal region of the protein sequence according to NP_005555.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 22kDa band observed in Human Kidney lysates (calculated MW of 22.6kDa according to NP_005555.2). Recommended concentration: 0.01-0.03µg/ml.
IHC: In paraffin embedded Human Spleen shows staining of cytoplasm in mostly binucleated cells, and of nucleoli in some of the non-dividing cells. Recommended concentration: 2-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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