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Code Name Applications Availability Size Tested species Price Grade  
EB13027 Goat Anti-MDH2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB13027 1
EB13027 (0.1µg/ml) staining of HeLa (A), HepG2 (B) and NIH3T3 (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB13027 2
EB13027 (0.01µg/ml) staining of Human (A), Mouse (B), Rat (C) and Pig (D) Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB13027 3
EB13027 (5µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: MDH2, malate dehydrogenase 2, NAD (mitochondrial), M-MDH, MDH, MGC:3559, MOR1, malate dehydrogenase, mitochondrial
Official Symbol: MDH2
Accession Number(s): NP_005909.2
Human GeneID(s): 4191
Non-Human GeneID(s): 17448 (mouse), 81829 (rat)

Immunogen

Peptide with sequence C-TRDDLFNTNAT, from the internal region of the protein sequence according to NP_005909.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 35kDa band observed in lysates of cell lines HeLa, HepG2 and NIH3T3 (calculated MW of 35.5kDa according to Human NP_005909.2 and 35.6kDa according to Mouse NP_032643.2 ). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. Approx 35kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 35.5kDa according to Human NP_005909.2 and 35.6kDa according to Mouse NP_032643.2, Rat NP_112413.2 and Pig NP_001231082.1 ). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human, PMID: 38609156.
IHC: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

Specific Reference

This antibody has been successfully used in WB on Human:
Michelle Noble, Aindrila Chatterjee, Thileepan Sekaran, Thomas Schwarzl, Matthias W Hentze
Cytosolic RNA binding of the mitochondrial TCA cycle enzyme malate dehydrogenase.
RNA. 2024 Jun 17;30(7):839-853.
PMID: 38609156

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