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Goat Anti-MEPCE Antibody (EB08073)

Code Name Applications Availability Size Tested species Price Grade  
EB08073 Goat Anti-MEPCE Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Rat $324.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB08073 1
EB08073 (1µg/ml) staining of Rat Testis lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08073 2
EB08073 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: MEPCE, methylphosphate capping enzyme, BCDIN3, FLJ20257, bin3, bicoid-interacting 3, bin3, bicoid-interacting 3, homolog
Official Symbol: MEPCE
Accession Number(s): NP_062552.2
Human GeneID(s): 56257

Immunogen

Peptide with sequence KQQRKFQYGNYCK, from the internal region of the protein sequence according to NP_062552.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx. 75kDa band observed in Rat Testis lysates (calculated MW of 74.4kDa according to Human NP_062552.2 and 75.3kDa according to Rat XP_222048.4). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Human for this particular batch.
IHC: In paraffin embedded Human Testis shows staining of the spermatids. Recommended concentration: 5-10µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat

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