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Goat Anti-Nalp10 / Nlrp10 (mouse) Antibody (EB11473)

Related Products: EBP11473 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11473 Goat Anti-Nalp10 / Nlrp10 (mouse) Antibody Pep-ELISA, IF, FC Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB11473 1
EB11473 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB11473 2
EB11473 Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: 6430548I20Rik, NACHT, leucine rich repeat and PYD containing 10, NACHT, LRR and PYD containing protein 10, NACHT, LRR and PYD domains-containing protein 10, NACHT/LRR/pyrin domain-containing protein 10, Nalp10, Napl10, neuronal apoptosis inhibitor protein 10, NLR family, pyrin domain containing 10, Nlrp10, PAN5, Pynod
Official Symbol: Nlrp10
Accession Number(s): NP_780741.1
Non-Human GeneID(s): 244202 (mouse), 293426 (rat)

Immunogen

Peptide with sequence NDLEENSFKTLKFH-C, from the N Terminus of the protein sequence according to NP_780741.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Mouse, Rat

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