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Goat Anti-NALP2 / PAN1 Antibody (EB09876)

Code Name Applications Availability Size Tested species Price Grade  
EB09876 Goat Anti-NALP2 / PAN1 Antibody Pep-ELISA, WB-Trf Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB09876 1
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NLRP2 with DYKDDDDK tag probed with EB09876 (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/5000) in lane C. Mock-transfected HEK293 probed with EB09876 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: CLR19.9, FLJ20510, NACHT, leucine rich repeat and PYD containing 2, NACHT, LRR and PYD containing protein 2, NALP2, NBS1, NLR family, pyrin domain containing 2, nucleotide-binding oligomerization domain, l, PAN1, PYPAF2, PYRIN-Containing APAF1-like, NLRP2
Official Symbol: NLRP2
Accession Number(s): NP_060322.1
Human GeneID(s): 55655

Immunogen

Peptide with sequence C-KNPQLIIDTEKHHP, from the C Terminus (near) of the protein sequence according to NP_060322.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: In transfected HEK293 transiently expressing full-length Human NLRP2 (myc and DYKDDDDK tagged), a band of approx. 150kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2ug/ml.

Purchase the +ve control! The transfected lysate used in QC is available for sale. Please email [email protected] to request datasheet, pricing and delivery information.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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