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Goat Anti-NDUFS8 Antibody (EB10193)

Related Products: EBP10193 - Immunizing Peptide, EBP10194 - Immunizing Peptide, Goat Anti-NDUFS8 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10193 Goat Anti-NDUFS8 Antibody Pep-ELISA, WB, IF, FC Yes 100µg specific antibody in 200µl Human, Rat $450.00 Elite_Plus

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10193 1
EB10193 (0.5µg/ml) staining of Rat Heart (A) and (2ug/ml) Rat Brain (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10193 2
EB10193 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB10193 3
EB10193 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: CI23KD, complex I-23kD, NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase), NADH dehydrogenase ubiquinone Fe-S 8, NADH-ubiquinone oxidoreductase 23 kDa subunit, TYKY, NDUFS8
Official Symbol: NDUFS8
Accession Number(s): NP_002487.1
Human GeneID(s): 4728
Non-Human GeneID(s): 225887 (mouse), 293652 (rat)

Immunogen

Peptide with sequence C-EAEIAANIQAD, from the internal region of the protein sequence according to NP_002487.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx. 25kDa band observed in Rat Heart and Brain lysates and in preliminary testing of Rat Kidney, Human Heart lysate and A549 cell lysate (calculated MW of 24kDa according to Rat : NP_001099792.1). Recommended concentration: 0.5-2µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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