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Goat Anti-neuronal pentraxin 1 Antibody (EB12221)

Code Name Applications Availability Size Tested species Price Grade  
EB12221 Goat Anti-neuronal pentraxin 1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $333.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
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EB12221 1
EB12221 (1µg/ml) staining of Human Frontal Cortex lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12221 2
EB12221 (5µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: NPTX1, neuronal pentraxin I, NP1, NP-I, neuronal pentraxin-1
Official Symbol: NPTX1
Accession Number(s): NP_002513.2
Human GeneID(s): 4884


Peptide with sequence C-ERVKIETALTSLHQR, from the internal region of the protein sequence according to NP_002513.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 50kDa band observed in Human Brain (Amygdala and Frontal Cortex) lysates (calculated MW of 47.1kDa according to NP_002513.2). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. Additional bands of unknown identity was also consistently observed at 38 and 14kDa. These bands were successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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