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Goat Anti-NMNAT3 (aa149-162) Antibody (EB11023)

Related Products: EBP08500 - Immunizing Peptide, EBP11023 - Immunizing Peptide, Goat Anti-NMNAT3 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB11023 Goat Anti-NMNAT3 (aa149-162) Antibody Pep-ELISA, IF, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11023 1
EB11023 Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB11023 2
EB11023 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: NMNAT3, nicotinamide nucleotide adenylyltransferase 3, PNAT3, NMN adenylyltransferase 3, NaMN adenylyltransferase 3, nicotinamide mononucleotide adenylyltransferase 3, nicotinate-nucleotide adenylyltransferase 3, pyridine nucleotide adenylyltransferase 3, NaMN adenylyltransferase 3, nicotinamide mononucleotide adenylyltransferase 3, nicotinamide nucleotide adenylyltransferase 3, nicotinate-nucleotide adenylyltransferase 3, NMN adenylyltransferase 3, PNAT3, pyridine nucleotide adenylyltransferase 3, NMNAT3
Official Symbol: NMNAT3
Accession Number(s): NP_835471.1; NP_001186976.1; NP_001307439.1; NP_001307440.1; NP_001307442.1
Human GeneID(s): 349565
Important Comments: This antibody is expected to recognize all reported isoforms

Immunogen

Peptide with sequence C-QHNIHLAKEPVQNE, from the internal region of the protein sequence according to NP_835471.1; NP_001186976.1; NP_001307439.1; NP_001307440.1; NP_001307442.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary testing showed a band at approx. 26kDa in Caco-2, A431 and A549 cell lysate at a concentration of 2ug/ml (calculated Mwt. of 24.1kDa according to NP_835471.1). Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in HEK293 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog

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