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Goat Anti-PARP3 Antibody (EB09029)

Related Products: EBP09029 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09029 Goat Anti-PARP3 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09029 1
EB09029 (3.75µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB09029 2
EB09029 (3.75µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PARP3, poly (ADP-ribose) polymerase family, member 3, ADPRT3, ADPRTL2, ADPRTL3, IRT1, hPARP-3, pADPRT-3, ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase)-like 2, ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase)-like 3, NAD+ ADP-ribosyltransferase 3, poly(ADP-ribose) polymerase 3, poly(ADP-ribose) synthetase-3
Official Symbol: PARP3
Accession Number(s): NP_001003931.2; NP_001003935.2
Human GeneID(s): 10039
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001003931.2; NP_001003935.2).

Immunogen

Peptide with sequence YEDYNCTLNQTNIEN , from the internal region of the protein sequence according to NP_001003931.2; NP_001003935.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Preliminary experiments in lysates of cell lines Daudi, HeLa and HepG2 gave no specific signal but low background (at antibody concentration up to 1µg/ml).
IHC: Paraffin embedded Human Liver and Kidney. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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