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Goat Anti-PAX8 (N Terminus) Antibody (EB05513)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB05513 | Goat Anti-PAX8 (N Terminus) Antibody | Pep-ELISA, WB, IHC |  |
100µg specific antibody in 200µl | Human | $423.00 |  |
|
Ordering Information for United States of America (change)Order direct from Everest
$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories. |
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EB05513 (1µg/ml) staining of Human Liver (A) and HEK293 cell (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05513 (4µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB05513 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Target Protein
Principal Names: PAX8, paired box gene 8, paired domain gene 8, paired box 8Official Symbol: PAX8
Accession Number(s): NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1
Human GeneID(s): 7849
Non-Human GeneID(s): 18510 (mouse), 81819 (rat)
Important Comments: This antibody is expected to recognise all five human isoforms (as represented by NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1)
Immunogen
Peptide with sequence PHNSIRSGHGGLN-C, from the N Terminus of the protein sequence according to NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:64000.Western blot: Approx 48kDa band observed in Human Liver lysates. An additional 35kDa bamd was also observed in some Liver and HEK293 cell lysates (calculated MW of 34.9kDa according to Human NP_039247.1 PAX8D and 48.2kDa according to Human NP_003457.1 PAXA). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 4-5µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Rat