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Goat Anti-Pituitary tumor-transforming 1 / Securin Antibody (EB06811)

Related Products: EBP06811 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06811 Goat Anti-Pituitary tumor-transforming 1 / Securin Antibody Pep-ELISA, IHC, IF, FC Yes 100µg specific antibody in 200µl Human $464.00 Elite_Plus

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EB06811 1
EB06811 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06811 2
EB06811 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06811 3
EB06811 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB06811 4
EB06811 (2.5µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06811 5
EB06811 (2.5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Target Protein

Principal Names: PTTG1, pituitary tumor-transforming 1, HGNC:9690, EAP1, HPTTG, PTTG, SECURIN, TUTR1, ESP1-associated protein 1, pituitary tumor-transforming protein 1, tumor-transforming protein 1
Official Symbol: PTTG1
Accession Number(s): NP_004210.1
Human GeneID(s): 9232
Important Comments: Likely to cross-react to PTTG2 and PTTG3

Immunogen

Peptide with sequence C-ILDEERELEKLFQ, from the internal region of the protein sequence according to NP_004210.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
IHC: Paraffin embedded Human Tonsil and Testis. Recommended concentration: 2.5µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog