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Goat Anti-PPP4C Antibody (EB09395)

Related Products: EBP09395 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09395 Goat Anti-PPP4C Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Rat $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09395 1
EB09395 (0.3µg/ml) staining of HEK293 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09395 2
EB09395 (0.3µg/ml) staining of Rat Spleen (A) and Lung (B) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09395 3
EB09395 (2.5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PPP4C, protein phosphatase 4 (formerly X), catalytic subunit, PP4, PPH3, PPX, Protein phosphatase 4, catalytic subunit
Official Symbol: PPP4C
Accession Number(s): NP_002711.1
Human GeneID(s): 5531
Non-Human GeneID(s): 171366 (rat)

Immunogen

Peptide with sequence C-PQETRGIPSKKP, from the C Terminus of the protein sequence according to NP_002711.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 35kDa band observed in HEK293, A431 and HeLa lysates and in Rat Spleen and Lung lysates (calculated MW of 35.1kDa according to NP_002711.1). Recommended concentration: 0.1-0.3µg/ml.
IHC: In paraffin embedded Human Prostate shows nuclear staining of secretory cells in the gland. Recommended concentration, 2-4µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Rat, Dog

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