Change country
Home

Goat Anti-PUM2 Antibody (EB06679)

Related Products: EBP06679 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06679 Goat Anti-PUM2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

Order offline

99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06679 1
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PUM2 with DYKDDDDK tag probed with EB06679 (0.5ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with EB06679 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
EB06679 2
EB06679 (10µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PUM2, PUMH2, PUML2, KIAA0235, pumilio homolog 2 (Drosophila), pumilio (Drosphila) homolog 2, FLJ36528, MGC138251, MGC138253, OTTHUMP00000200539, pumilio homolog 2
Official Symbol: PUM2
Accession Number(s): NP_056132.1
Human GeneID(s): 23369

Immunogen

Peptide with sequence C-DQNRDLKQGDDDDSK, from the internal region of the protein sequence according to NP_056132.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: In transfected HEK293 transiently expressing full-length Human PUM2 (myc and DYKDDDDK tagged), a band of approx. 140kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration 0.3-0.5µg/ml.
IHC: Paraffin embedded Human Testis. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Cow

Product Reviews

Please login to review this product.