Choose your country here (United States of America)
Search
Type in product code or name:
Goat Anti-Septin 1 / SEPT1 Antibody (EB07412)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB07412 | Goat Anti-Septin 1 / SEPT1 Antibody | Pep-ELISA, WB, IHC |  |
100µg specific antibody in 200µl | Human | $423.00 |  |
|
Ordering Information for United States of America (change)Order direct from Everest |
||||||||
EB07412 (1µg/ml) staining of HEK293 (A) and (2ug/ml) MOLT-4 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence
EB07412 (6µg/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB07412 Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
Target Protein
Principal Names: SEPT1, septin 1, DIFF6, LARP, MGC20394, PNUTL3, SEP1, differentiation 6 (deoxyguanosine triphosphate triphosphohydrolase), serologically defined breast cancer antigen NY-BR-24Official Symbol: SEPT1
Accession Number(s): NP_443070.6
Human GeneID(s): 1731
Non-Human GeneID(s): 54204 (mouse), 293507 (rat)
Immunogen
Peptide with sequence C-GARDRASRSKLSRQ, from the internal region of the protein sequence according to NP_443070.6.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Approx 42-45kDa band observed in lysates of cell lines HEK293 and MOLT-4 (calculated MW of 42.4kDa according to NP_443070.6). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour. at room temperature.
IHC: Paraffin embedded Human Spleen. Recommended concentration: 6-8µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Rat, Dog