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Goat Anti-SET / I2 alpha PP2A Antibody (EB05148)

Related Products: EBP05148 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05148 Goat Anti-SET / I2 alpha PP2A Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05148 1
EB05148 (0.3µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05148 2
EB05148 (0.01µg/ml) staining of Daudi (A) and Molt4 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05148 3
EB05148 (7µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB05148 4
EB05148 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: SET, SET nuclear oncogene, 2PP2A, I2PP2A, IGAAD, IPP2A2, PHAPII, TAF-I, TAF-IBETA, LA-DR-associated protein II, OTTHUMP00000022286, SET translocation (myeloid leukemia-associated), Template-Activating Factor-I, chromatin remodelling factor, inhibitor of granzyme A-activated DNase, inhibitor-2 of protein phosphatase-2A, protein phosphatase type 2A inhibitor, template-activating factor I, HLA-DR-associated protein II
Official Symbol: SET
Accession Number(s): NP_003002.2
Human GeneID(s): 6418
Important Comments: This antibody is expected to recognize isoform 2 (NP_003002.2).

Immunogen

Peptide with sequence SAPAAKVSKKELNS-C, from the N Terminus of the protein sequence according to NP_003002.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 38kDa band observed in lysates of Human Kidney lysates and of cell lines Daudi and Molt4 (calculated MW of 32.1kDa according to NP_003002.2). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 7µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Cow

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