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Goat Anti-SMC5 Antibody (EB09424)

Related Products: EBP09424 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09424 Goat Anti-SMC5 Antibody Pep-ELISA, IF, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09424 1
EB09424 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing speckled nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09424 2
EB09424 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: SMC5, structural maintenance of chromosomes 5, KIAA0594, SMC5L1, OTTHUMP00000063367, SMC5 protein, SMC5 structural maintenance of chromosomes 5-like 1
Official Symbol: SMC5
Accession Number(s): NP_055925.2
Human GeneID(s): 23137
Non-Human GeneID(s): 226026 (mouse), 293967 (rat)

Immunogen

Peptide with sequence C-TSKHLEHKDNELRQ, from the Internal region of the protein sequence according to NP_055925.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary testing showed bands at approx 100+150kDa in HEK293 nuclear lysates after 1µg/ml antibody staining (calculated MW of 129kDa according to NP_055925.2). These bands were successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow, Dog

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