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Goat Anti-SMUG1 Antibody (EB06321)

Related Products: EBP06321 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06321 Goat Anti-SMUG1 Antibody Pep-ELISA, IF, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06321 1
EB06321 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06321 2
EB06321 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06321 3
EB06321 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: SMUG1, single-strand selective monofunctional uracil DNA glycosylase, FDG, HMUDG, UNG3, single-strand-selective monofunctional uracil-DNA glycosylase 1, MGC104370
Official Symbol: SMUG1
Accession Number(s): NP_055126.1; NP_001230718.1; NP_001338187.1; NP_001338190.1
Human GeneID(s): 23583

Immunogen

Peptide with sequence PQAFLLGSIHEPA-C, from the N Terminus of the protein sequence according to NP_055126.1; NP_001230718.1; NP_001338187.1; NP_001338190.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary testing showed a band at approx 19kDa in lysate of cell line HeLa after 0.1µg/ml antibody staining (calculated MW of 19.6kDa according to NP_001230718.1);. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 and U205 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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