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Goat Anti-SPFH2 / ERLIN2 Antibody (EB06896)

Related Products: EBP06896 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06896 Goat Anti-SPFH2 / ERLIN2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06896 1
EB06896 staining (1µg/ml) of Human Breast lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
EB06896 2
EB06896 (3.75µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ERLIN2, SPFH2, ER lipid raft associated 2, SPFH domain family, member 2, HGNC:1356, C8orf2, MGC87072, chromosome 8 open reading frame 2, Erlin-2, MGC87072
Official Symbol: ERLIN2
Accession Number(s): NP_009106.1
Human GeneID(s): 11160
Non-Human GeneID(s): 290823 (rat)
Important Comments: This antibody is expected to recognise isoform 1 (NP_009106) only.

Immunogen

Peptide with sequence C-EDEPLETATKEN, from the C Terminus of the protein sequence according to NP_009106.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 43kDa band observed in Human Breast lysates and in lysates of Human Breast Cancer (calculated MW of 37.8kDa according to NP_009106.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pearce et al, J Biol Chem. 2007 Jul 13;282(28):20104-15.; PMID: 17502376). Recommended concentration: 0.3-1µg/ml.
IHC: Paraffin embedded Human Breast. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Rat

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