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Goat Anti-SRGAP2 Antibody (EB09775)

Related Products: EBP09775 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09775 Goat Anti-SRGAP2 Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09775 1
EB09775 (0.1µg/ml) staining of Rat Cortical Neuron lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Ms. Ya-Jing Mi and Dr.Wei-Lin Jin, Institute of Neurosciences, Shanghai Jiao Tong University.
EB09775 2
HEK293 overexpressing Human srGAP2 and probed with EB09775 at 2.5ug/ml in the right panel. Data kindly provided by Ms. Ya-Jing Mi and Dr.Wei-Lin Jin, Institute of Neurosciences, Shanghai Jiao Tong University.
Click here to download PDF datasheet.

Target Protein

Principal Names: SRGAP2, FNBP2, formin binding protein 2, KIAA0456, SLIT-ROBO Rho GTPase activating protein 2, srGAP3
Official Symbol: SRGAP2
Accession Number(s): NP_056141.2
Human GeneID(s): 23380
Non-Human GeneID(s): 14270 (mouse), 360840 (rat)
Important Comments: This antibody is expected to recognize isoform a (NP_056141.2).

Immunogen

Peptide with sequence C-KQEDRQTPRSPDST, from the internal region of the protein sequence according to NP_056141.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 124kDa band observed in lysates of cell line HeLa and in rat primary culture cortical neurons at Day 4 (calculated MW of 121kDa according to human NP_056141.2 but the rat protein sequence is stlll provisional). Recommended concentration: 0.1-0.3µg/ml.

Immunofluorescence: Transfected HEK293 transiently expressing GFP-tagged Human srGAP2 with the primary antibody stained in the red channel (Confocal IF).

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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