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Code Name Applications Availability Size Tested species Price Grade  
EB07379 Goat Anti-STAT3 Antibody Pep-ELISA, WB, IHC, FC Yes 100µg specific antibody in 200µl Human, Mouse $450.00 Elite_Plus

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07379 1
EB07379 (3µg/ml) staining of LNCaP cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07379 2
EB07379 (0.5µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07379 3
EB07379 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07379 4
EB07379 Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: STAT3, signal transducer and activator of transcription 3 (acute-phase response factor), APRF, FLJ20882, MGC16063, DNA-binding protein APRF, acute-phase response factor, signal transducer and activator of transcription 3
Official Symbol: STAT3
Accession Number(s): NP_644805.1; NP_003141.2; NP_998827.1
Human GeneID(s): 6774
Non-Human GeneID(s): 20848 (mouse), 25125 (rat)
Important Comments: This antibody is expected to recognise all three reported isoforms (NP_644805.1, NP_003141.2 and NP_998827.1).

Immunogen

Peptide with sequence CRPESQEHPEADP, from the internal region of the protein sequence according to NP_644805.1; NP_003141.2; NP_998827.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 85kDa band observed in lysates of cell line LNCaP, and approx. 90kDa in Mouse Heart lysates (calculated MW of 83.1kDa according to Human NP_998827.1 and 88.1kDa according to Mouse : NP_998824.1). Recommended concentration: 0.5-3ug/ml. Primary incubation 1 hour. at room temperature.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog

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