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Goat Anti-STK39 / SPAK Antibody (EB09416)

Related Products: EBP09416 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09416 Goat Anti-STK39 / SPAK Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09416 1
EB09416 (0.3µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09416 2
EB09416 (6µg/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB09416 3
EB09416 Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: STK39, serine threonine kinase 39 (STE20/SPS1 homolog, yeast), DCHT, DKFZp686K05124, PASK, SPAK, Ste20-like protein kinase, proline-alanine-rich STE20-related kinase, small intestine SPAK-like kinase
Official Symbol: STK39
Accession Number(s): NP_037365.2
Human GeneID(s): 27347
Non-Human GeneID(s): 53416 (mouse), 54348 (rat)

Immunogen

Peptide with sequence C-SQEKSRRVKEENPE, from the internal region of the protein sequence according to NP_037365.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 65kDa band observed in Human Brain (Cerebellum) lysate (calculated MW of 59.5kDa according to NP_037365.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1µg/ml.
IHC: Paraffin embedded Human Testis. Recommended concentration: 6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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