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Code Name Applications Availability Size Tested species Price Grade  
EB07594 Goat Anti-STS2 / TULA (internal) Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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EB07594 1
EB07594 (2.5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: TULA, STS2 CBL-interacting protein 4, UBASH3A, ubiquitin associated and SH3 domain containing, A, T-cell ubiquitin ligand protein, Suppressor of T-cell receptor signaling 2, CLIP4
Official Symbol: UBASH3A
Accession Number(s): NP_061834.1; NP_001001895.1
Human GeneID(s): 53347
Important Comments: This antibody is expected to recognize both reported isoforms (NP_061834.1 and NP_001001895.1).

Immunogen

Peptide with sequence QQCSTPDGKYYRPD, from the internal region of the protein sequence according to NP_061834.1; NP_001001895.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave an approx 48kDa band in lysates of B-cell line Daudi after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 74.1kDa according to NP_061834.1 and 69.8kDa according to NP_001001895.1. The 48kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Spleen shows cytoplasm staining of T-cells eccentrically gathered around an artery. Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog

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