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Goat Anti-TIGD1 / EEYORE Antibody (EB06265)

Related Products: EBP06265 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06265 Goat Anti-TIGD1 / EEYORE Antibody Pep-ELISA, IF Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06265 1
EB06265 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: TIGD1, EEYORE, tigger transposable element derived 1, jerky (mouse) homolog-like, hypothetical protein LOC200765, jerky homolog-like
Official Symbol: TIGD1
Accession Number(s): NP_663748.1
Human GeneID(s): 200765

Immunogen

Peptide with sequence C-PAKRVRLTEGSD, from the C Terminus of the protein sequence according to NP_663748.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments gave no signal but low background in Human Brain and Kidney extracts at up to 1µg/ml.

Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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