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Goat Anti-TNNI3 (aa117-127) Antibody (EB11958)

Related Products: EBP11957 - Immunizing Peptide, EBP11958 - Immunizing Peptide, Goat Anti-TNNI3 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB11958 Goat Anti-TNNI3 (aa117-127) Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig $411.00 Elite

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ARP American Research Products, Inc
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EB11958 1
EB11958 (0.1µg/ml) staining of Human (A), (0.03µg/ml) Mouse (B), (0.1µg/ml) Rat (C) and Pig (D) Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11958 2
EB11958 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB11958 3
EB11958 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: TNNI3, troponin I type 3 (cardiac), CMD1FF, CMD2A, CMH7, RCM1, TNNC1, cTnI, troponin I, cardiac muscle
Official Symbol: TNNI3
Accession Number(s): NP_000354.4
Human GeneID(s): 7137
Non-Human GeneID(s): 21954 (mouse), 29248 (rat)

Immunogen

Peptide with sequence C-KVTKNITEIAD, from the internal region of the protein sequence according to NP_000354.4.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 25+26kDa doublet band observed in Human Heart, approx 24+27kDa in Mouse Heart, approx 25kDa in Rat Heart and approx 27kDa in Pig Heart lysates (calculated MW of 24kDa according to Human NP_000354.4, and Pig NP_001092069.1 and 24.3kDa according to Mouse NP_033432.1 and Ra tNP_058840.1 ). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and HeLa cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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