Goat Anti-AADACL1 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB08327
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
arylacetamide deacetylase-like 1|AADACL1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the Endoplasmic reticulum of A431 and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_065843.3; NP_001139748.2; NP_001139749.1
Blocking Peptide
EBP08327
Immunogen
Peptide with sequence C-RTRNSYIKWLDQN, from the C Terminus of the protein sequence according to NP_065843.3; NP_001139748.2; NP_001139749.1.
Peptide Sequence
C-RTRNSYIKWLDQN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Dog, Cow
Reactive Species
Human
Human Gene ID
57552
Mouse Gene ID
320024
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:1000.
Western Blot
Preliminary experiments gave an approx 26-28kDa band in A431 cell lysates and in Human Hippocampus lysates after 1µg/ml antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. Please note that we currently cannot find an explanation in the literature for the band, given the calculated size of 31.2kDa according to NP_001139749.1
Application Type
Pep-ELISA, IF, FC

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