Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2|alpha-2-plasmin inhibitor|PLI|API|ALPHA-2-PI|AAP|A2AP|serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2|SERPINF2 | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_000925.2; NP_001159393.1 | |
Blocking Peptide EBP08777 | |
Immunogen Peptide with sequence C-KDFLQSLKGFPRGDK, from the internal region of the protein sequence according to NP_000925.2; NP_001159393.1. | |
Product Comments This antibody is expected to recognise both reported isoforms (NP_000925.2; NP_001159393.1). Reported variants represent identical protein (NP_000925.2; NP_001159392.1). | |
Peptide Sequence C-KDFLQSLKGFPRGDK | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human | |
Reactive Species Human | |
Human Gene ID 5345 | |
Product Grade  | |
IHC Results In paraffin embedded Human Liver shows spotty cytoplasm staining in hepatocytes. Recommended concentration: 3-5µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:16000. | |
Western Blot Approx 70kDa band observed in lysates of cell line HepG2 (calculated MW of 54.6kDa according to NP_000925.2). The observed molecular weight is observed by other sources. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. This antibody has been successfully used in WB on Human: Abdul S et al. (2020) PMID: 32034861. | |
Application Type Pep-ELISA, WB, IHC, IF, FC |
Goat Anti-Alpha2-antiplasmin Antibody
$464.00
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Selected References [{"pmid": 32034861, "intro": "This antibody has been successfully used in Western blot on Human:", "title": "On the localization of the cleavage site in human alpha-2-antiplasmin, involved in the generation of the non-plasminogen binding form.", "author": "Abdul S, Dekkers DHW, Ariëns RAS, Leebeek FWG, Rijken DC, Uitte de Willige S", "journal": "J Thromb Haemost. 2020 Feb 8"}] |