Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names glutamyl aminopeptidase|CD249|gp160|APA|HGNC:3355|glutamyl aminopeptidase (aminopeptidase A)|ENPEP|aminopeptidase A | |
Accession ID NP_001968.3 | |
Blocking Peptide EBP06798 | |
Immunogen Peptide with sequence C-EQYQKTSLAQEKEK, from the internal region of the protein sequence according to NP_001968.3. | |
Peptide Sequence C-EQYQKTSLAQEKEK | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Pig, Cow | |
Reactive Species Human, Rat | |
Human Gene ID 2028 | |
Mouse Gene ID 13809 | |
Product Grade  | |
IHC Results In paraffin embedded Human Kidney shows staining of basement membrane and the mesangium in the glomerulus. Recommended concentration: 3-5µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:64000. | |
Western Blot Approx 170kDa band observed in Human Brain and Testis lysates (calculated MW of 109kDa according to NP_001968.2). Primary incubation was for 1 hour.The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ando et al, J Clin Endocrinol Metab. 2002 May;87(5):2303-9; PMID: 11994381). Recommended concentration: 1-3µg/ml. This antibody has been successfully used in WB on Rat: PMID: 28399178 and 25470983. | |
Application Type Pep-ELISA, WB, IHC |
Goat Anti-Aminopeptidase A Antibody
$423.00
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Selected References [{"pmid": 25470983, "intro": "This antibody has been successfully used in WB on Rat:", "title": "Immunological detection of glutamyl aminopeptidase in urine samples from cisplatin-treated rats.", "author": "Montoro-Molina S, Quesada A, Zafra-Ruiz PV, O'Valle F, Vargas F, de Gracia Mdel C, Osuna A, Wangensteen R", "journal": "Proteomics Clin Appl. 2015 Jun;9(5-6):630-5. "}, {"pmid": 28399178, "intro": "This antibody has been successfully used in WB on Rat:", "title": "Glutamyl aminopeptidase in microvesicular and exosomal fractions of urine is related with renal dysfunction in cisplatin-treated rats.", "author": "Quesada A, Segarra AB, Montoro-Molina S, de Gracia MD, Osuna A, O'Valle F, Gómez-Guzmán M, Vargas F, Wangensteen R.", "journal": "PLoS One. 2017 Apr 11;12(4):e0175462"}] |