Goat Anti-APOL3 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB08917
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
apolipoprotein L3|apolipoprotein L-III|TNF-inducible protein CG12-1|OTTHUMP00000028913|CG12-1|APOLIII|apolipoprotein L, 3|APOL3
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of Jurkat cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
Accession ID
NP_663615.1, NP_055164.1, NP_663616.1; NP_001380516.1; NP_001380534.1
Blocking Peptide
EBP08917
Immunogen
Peptide with sequence ELTQIYQRLNPCHTH, from the C Terminus of the protein sequence according to NP_663615.1, NP_055164.1, NP_663616.1; NP_001380516.1; NP_001380534.1.
Product Comments
This antibody is expected to recognize isoforms 1 -5.
Peptide Sequence
ELTQIYQRLNPCHTH
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
80833
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:1000.
Western Blot
Approx. 38kDa band observed in lysates of cell lines Jurkat and A549 (calculated MW of 36.5kDa according to NP_055164.1). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, FC, IF

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