Goat Anti-ARF1 (variants 1-4) Antibody

$423.00

Category: Primary Antibodies
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SKU: EB07557
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
ADP ribosylation factor 1|ARF 1|ADP-ribosylation factor 1|ARF1
Usage Summary
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
Accession ID
NP_001019397.1; NP_001649.1; NP_001019398.1; NP_001019399.1
Blocking Peptide
EBP07557
Immunogen
Peptide with sequence C-EGLDWLSNQLRNQK, from the C Terminus of the protein sequence according to NP_001019397.1; NP_001649.1; NP_001019398.1; NP_001019399.1.
Product Comments
This antibody is expected to recognize all four reported isoforms.
Peptide Sequence
C-EGLDWLSNQLRNQK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog
Reactive Species
Human, Mouse, Rat, Dog
Human Gene ID
375, 376
Mouse Gene ID
11840
Rat Gene ID
64310
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:8000.
Western Blot
Approx 18kDa band observed in lysates of cell lines A431, HeLa, MCF7, NIH3T3, KNRK and MDCK (calculated MW of 20.7kDa according to Human NP_001649.1, Mouse NP_031502.1 and Rat NP_071963.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, FC

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