Goat Anti-ARP1 homolog A Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

ARP1 actin-related protein 1 homolog A, centractin alpha|FLJ55002|FLJ52800|FLJ52695|CTRN1|ARP1 (actin-related protein 1, yeast) homolog A (centractin alpha)|centrosome-associated actin homolog|ARP1, yeast homolog A|centractin alpha|actin-RPV|ARP1 actin-related protein 1 homolog A, centractin alpha (yeast)|ARP1|ACTR1A

Immunofluorescence: A customer reported cytoplasmic staining on A431 cells at a primary ab concentration of 10ug/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

NP_005727.1

EBP06349

Peptide with sequence C-YEEDGARSIHRKT, from the C Terminus of the protein sequence according to NP_005727.1.

This epitope was selected to minimise the chance of cross-reactivity with ACTR1B (YEEDGSRAIHRKT).

C-YEEDGARSIHRKT

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog, Pig, Cow

Human, Mouse, Rat, Dog

10121

54130

Antibody detection limit dilution 1:64000.

Approx. 42-45kDa band observed in lysates of cell lines A431 HEK293, HeLa, MCF7, NIH3T3, KNRK and MDCK, and in preliminary testing of Human and Mouse Adrenal gland and Mouse Stomach lysate (calculated MW of 42.6kDa according to Human NP_005727.1, Mouse : NP_058556.1, Rat NP_001099834.1 and Dog NP_001003164.1). Recommended concentration: 0.1-2µg/ml. Primary incubation 1 hour at room temperature.

Pep-ELISA, WB, FC