Goat Anti-BRG1 / SMARCA4 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB06353
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
global transcription activator homologous sequence|mitotic growth and transcription activator|sucrose nonfermenting-like 4|homeotic gene regulator|BRM/SWI2-related gene 1|brahma protein-like 1|nuclear protein GRB1|SNF2-like 4|SNF2-beta|SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4|SNF2-BETA|hSNF2b|hSNF2B|SNF2LB|SNF2L4|BAF190|BRG1|SMARCA4
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_001122321.1; NP_003063.2; NP_001122317.1; NP_001122318.1; NP_001122319.1; NP_001122320.1
Blocking Peptide
EBP06353
Immunogen
Peptide with sequence STPDPPLGGTPRP-C, from the N Terminus of the protein sequence according to NP_001122321.1; NP_003063.2; NP_001122317.1; NP_001122318.1; NP_001122319.1; NP_001122320.1.
Product Comments
This antibody is expected to recognize all reported isoforms (NP_001122321.1; NP_003063.2; NP_001122317.1; NP_001122318.1; NP_001122319.1; NP_001122320.1). Reported variants NP_003063.2 and NP_001122316.1 represent identical protein.
Peptide Sequence
STPDPPLGGTPRP-C
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog
Reactive Species
Human
Human Gene ID
6597
Mouse Gene ID
20586
Rat Gene ID
171379
Product Grade
IHC Results
Paraffin embedded Human Small Intestine, Tonsil and Prostate. Recommended concentration: 3.75µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx. 200-250kDa band observed in nuclear lysates of cell line Jurkat (calculated MW of 185kDa according to NP_003063.2). This molecular weight is observed by other commercial sources, and was blocked by incubation with the immunizing peptide. Recommended concentration: 0.01-0.03ug/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, IHC, FC

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