Goat Anti-C14orf68 Antibody

$423.00

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SKU: EB07604
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
C14orf68|chromosome 14 open reading frame 68|HDMCP|HMFN1655|hepatocellular carcinoma-downregulated mitochondrial carrier protein
Accession ID
NP_997000.2
Blocking Peptide
EBP07604
Immunogen
Peptide with sequence C-RYGNPDAKPTKAD, from the internal region of the protein sequence according to NP_997000.2.
Peptide Sequence
C-RYGNPDAKPTKAD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
283600
Product Grade
IHC Results
In paraffin embedded Human Liver shows staining of nucleoli in select hepatocytes. Recommended concentration, 2.5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Preliminary experiments gave an approx 45kDa band in Human Liver lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.4kDa according to NP_997000.2. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA, IHC