Goat Anti-calbindin D28 Antibody

$423.00

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SKU: EB11759
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
vitamin D-dependent calcium-binding protein, avian-type|RTVL-H protein|OTTHUMP00000225441|OTTHUMP00000166027|D-28K|calbindin D28|calbindin 1, 28kDa|calbindin 1, (28kD)|calbindin|CALB1|CALB
Accession ID
NP_004920.1
Blocking Peptide
EBP11759
Immunogen
Peptide with sequence C-KTFVDQYGQRDDGK, from the internal region of the protein sequence according to NP_004920.1.
Product Comments
Immunizing peptide overlaps EF hand 2, but not any calcium binding site.
Peptide Sequence
C-KTFVDQYGQRDDGK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Rat, Dog, Pig, Cow
Reactive Species
Human, Rat
Human Gene ID
793
Rat Gene ID
83839
Product Grade
IHC Results
Paraffin embedded Human Kidney. Recommended concentration: 5-7µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx. 26kDa band observed in Human and Rat Kidney lysates and in Rat Brain lysates (calculated MW of 30.0kDa according to Human NP_004920.1and Rat NP_114190.1). The observed molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC
Selected References
[{"pmid": 0, "intro": "This antibody (previous batch) has been successfully used in the following paper:", "title": "Comparative Renal Development between Spiny Mouse (Acomys cahirinus) and House Mouse (Mus musculus)", "author": "Azad, Tanzina", "journal": "http://hdl.handle.net/10138/594531 (2025)"}]