Goat Anti-Calcipressin-1 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

calcium and| calcipressin-1| OTTHUMP00000214670| OTTHUMP00000214669| OTTHUMP00000108622| OTTHUMP00000108621| Down syndrome candidate region 1| RCN1| MCIP1| DSCR1| DSC1| CSP1| ADAPT78| regulator of calcineurin 1|RCAN1

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

NP_004405.3; NP_981962.1; NP_981963.1; NP_001272320.2; NP_001272318.1; NP_001317945.1

EBP10693

Peptide with sequence C-HIGSSHLAPPNPD, from the internal region of the protein sequence according to NP_004405.3; NP_981962.1; NP_981963.1; NP_001272320.2; NP_001272318.1; NP_001317945.1.

This antibody is expected to recognize all reported isoforms a to f.

C-HIGSSHLAPPNPD

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog, Cow

Human, Mouse

1827

54720

266766

Antibody detection limit dilution 1:32000.

Approx. 40kDa band was observed in Human Cerebellum lysates (calculated MW of 31.9kDa according to NP_001272320.2). This molecular weight is observed by other commercial sources, and was blocked by incubation with the immunizing peptide. Recommended concentration: 1-1.5ug/ml. Primary incubation 1 hour at room temperature. Approx. 40kDa band corresponding to isoform 1L, was observed in Wild-type Mouse Brain lysates, which is not present in the KO mouse. Additional 55kDa bands were consistently observed in both the WT and KO Mouse, and are therefore a non-specific signal. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. Data kindly provided by Dana Crawford, PhD, Albany Medical College, NY, USA.

Pep-ELISA, WB, IF, FC