Goat Anti-CAMK2 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

calcium/calmodulin-dependent protein kinase type II alpha chain|calcium/calmodulin-dependent protein kinase IIA|calcium/calmodulin-dependent protein kinase II alpha-B subunit|OTTHUMP00000165788|OTTHUMP00000165787|CaMK-II alpha subunit|CaM-kinase II alpha chain|CaM kinase II alpha subunit|KIAA0968|CAMKA|calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha|CAMK2A

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of Neuro2a cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of Neuro2a cells. Recommended concentration: 10ug/ml.

NP_057065.2; NP_001280099.1

EBP09376

Peptide with sequence C-PRTAQSEETRVWHR, from the internal region of the protein sequence according to NP_057065.2; NP_001280099.1.

This antibody is expected to recognize both reported isoforms (NP_057065.2 (CAMK2A) and NP_001280099.1 (CAMK2B).

C-PRTAQSEETRVWHR

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat

Human, Mouse, Rat

815, 816

12322

25400

Antibody detection limit dilution 1:128000.

Approx 48-50+60kDa bands observed in Human Cerebral Cortex and Mouse and Rat Brain lysates, corresponding to CAMK2alpha and CAMK2beta respectively and approx. 60kDa in Human Cerebellum lysates (calculated MW of 55.3kDa according to Human NP_057065.2 and 54.1kDa according to Mouse NP_001390241.1 and Rat NP_037052.1 subunit Alpha and 60.4kDa according to Human NP_001280099.1, Mouse NP_031621.3 and Rat NP_068507.2 subunit beta). These molecular weights are routinely observed by other sources, and were successfully blocked by incubation with the immunizing peptide. Recommended concentration 0.1-1ug/ul. Primary incubation 1 hour at room temperature.

Pep-ELISA, WB, IF, FC