Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names cannabinoid receptor 1|OTTHUMP00000016839|OTTHUMP00000016838|CB1R|RP1-23D17.1|central cannabinoid receptor|HGNC:2159, CANN6, CB-R, CB1, CB1A, CB1K5, CNR|CNR1|cannabinoid receptor 1 (brain) | |
Accession ID NP_057167.2; NP_001153732.1; NP_001153731.1; NP_001153730.1; NP_001153698.1; NP_149421.1 | |
Blocking Peptide EBP06945 | |
Immunogen Peptide with sequence C-HTSEDGKVQVTRPDQ, from the internal region of the protein sequence according to NP_057167.2; NP_001153732.1; NP_001153731.1; NP_001153730.1; NP_001153698.1; NP_149421.1. | |
Product Comments This antibody is expected to recognise both reported isoforms. Isoform a (NP_057167.2; NP_001153732.1; NP_001153731.1; NP_001153730.1; NP_001153698.1) and isoform b (NP_149421.1). | |
Peptide Sequence C-HTSEDGKVQVTRPDQ | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog | |
Reactive Species Human | |
Human Gene ID 1268 | |
Mouse Gene ID 12801 | |
Rat Gene ID 25248 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:32000. | |
Western Blot Approx 55kDa (non-glycosylated) and 65kDa (glycosylated) bands observed in lysates of cell line HeLa (calculated MW of 52.9kDa according to NP_057167.2;). Both bands were successfully blocked by incubation with the immunizing peptide, Recommended concentration: 0.5-2µg/ml. Primary incubation 1 hour at room temperature.
This antibody has been successfully used in Western blot on Human: Fügedi G et al. (2014) PMID: 25444073. | |
Application Type Pep-ELISA, WB |
Goat Anti-Cannabinoid Receptor 1 Antibody
$423.00
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Selected References [{"pmid": 25444073, "intro": "This antibody has been successfully used in Western blot on Human:", "title": "Increased placental expression of cannabinoid receptor 1 in preeclampsia: an observational study.", "author": "Fügedi G, Molnár M, Rigó J Jr, Schönléber J, Kovalszky I, Molvarec A.", "journal": "BMC Pregnancy Childbirth. 2014 Dec 2;14:395"}] |