Goat Anti-CAPG Antibody

$423.00

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SKU: EB12421
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
macrophage-capping protein|macrophage capping protein|gelsolin-like capping protein|actin-regulatory protein CAP-G|actin regulatory protein CAP-G|MCP|AFCP|capping protein (actin filament), gelsolin-like|CAPG
Usage Summary

Flow Cytometry: Flow cytometric analysis of THP-1 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_001738.2; NP_001243069.1; NP_001307663.1
Blocking Peptide
EBP12421
Immunogen
Peptide with sequence C-NIRATERALNWDS, from the internal region of the protein sequence according to NP_001738.2; NP_001243069.1; NP_001307663.1.
Product Comments
This antibody is expected to recognize all reported isoform (NP_001738.2; NP_001243069.1, NP_001307663.1. Reported variants represent identical protein: NP_001243068.1, NP_001738.2.
Peptide Sequence
C-NIRATERALNWDS
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Dog
Reactive Species
Human
Human Gene ID
822
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx. 38kDa band observed in lysates of cell lines THP-1, U937 and A431, and in Human Spleen lysates and approx. 37kDa in Human Tonsil lysates (calculated MW of 38.5kDa according to NP_001738.2 and 36.9kDa according to NP_001243069.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.

Positive Control: A batch specific positive control lysate is available for this product. Please contact [email protected] for availability.

Application Type
Pep-ELISA, WB, FC