Goat Anti-CARD6 Antibody

$423.00

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SKU: EB05922
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
CARD-containing inhibitor of Nod1 and Cardiak-induced NF-kB activation|CINCIN1|caspase recruitment domain protein 6|caspase recruitment domain family, member 6|CARD6
Accession ID
NP_115976.2
Blocking Peptide
EBP05922
Immunogen
Peptide with sequence ATESTPSEIIERER-C, from the N Terminus of the protein sequence according to NP_115976.2.
Peptide Sequence
ATESTPSEIIERER-C
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
84674
Product Grade
IHC Results
In paraffin embedded Human Pancreas shows variable staining of cytoplasm in acinar cells. Recommended concentration, 5-10µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx. 45-50kDa and 25-30kDa bands observed in A549, Human Heart and Human Skeletal Muscle lysates at 1ug of primary. Please note that the bands we observe are different from the predicted size of approx. 119kDa according to NP_115976. Currently we cannot find an explanation for this in the literature. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any splice variants/modified forms been reported?
Application Type
Pep-ELISA, IHC