Goat Anti-CD68 Antibody

$464.00

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SKU: EB12699
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
scavenger receptor class D, member 1|macrosialin|macrophage antigen CD68|CD68 antigen|SCARD1|LAMP4|GP110|CD68 molecule|CD68
Usage Summary
Flow Cytometry: Flow cytometric analysis of U937 cells. Recommended concentration: 10ug/ml.
Accession ID
NP_001242.2; NP_001035148.1
Blocking Peptide
EBP12699
Immunogen
Peptide with sequence PNKTKVQGSCEGAH, from the internal region of the protein sequence according to NP_001242.2; NP_001035148.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_001242.2; NP_001035148.1).
Peptide Sequence
PNKTKVQGSCEGAH
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
968
Product Grade
IHC Results
Paraffin embedded Human Spleen. Recommended concentration: 5-6µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx. 100-110kDa band observed in lysates of cell line U937 and in preliminary testing of THP-1 cell lysate (calculated MW of 37.4kDa according to NP_001242.2). The observed molecular weight is routinely reported, and corresponds to the glycosylated form. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. Positive Control: A batch specific positive control lysate is available for this product. Please contact [email protected] for availability.
Application Type
Pep-ELISA, WB, IHC, FC